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pcr thermal cycler dice touch  (TaKaRa)


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    Structured Review

    TaKaRa pcr thermal cycler dice touch
    Pcr Thermal Cycler Dice Touch, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 4069 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thermal+cycler+dice+touch/Takara+PCR+Thermal+Cycler+Dice/pm42031269-71-7-12
    Average 98 stars, based on 4069 article reviews
    pcr thermal cycler dice touch - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund's adjuvant-induced inflammatory pain mouse model.
    Article Snippet: .. Polymerase chain reaction (PCR) was performed using a TaKaRa Thermal Cycler Dice Touch (Shiga, Japan) according to the following program: predenaturation at 94 °C for 3 min; 40 cycles for MT3 with denaturation at 94 °C for 30 s, annealing at 63 °C for 1 min, and extension at 72 °C for 1 min; followed by final extension at 70 °C for 3 min. ..

    Article Title: Genome survey and microsatellite marker development in the Antarctic Eaton’s skate, Bathyraja eatonii (Rajiformes, Arhynchobatidae)
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Metallothionein-3-mediated intracellular zinc mediates antioxidant and anti-inflammatory responses in the complete Freund’s adjuvant-induced inflammatory pain mouse model
    Article Snippet: .. Polymerase chain reaction (PCR) was performed using a TaKaRa Thermal Cycler Dice Touch (Shiga, Japan) according to the following program: pre-denaturation at 94 °C for 3 min; 40 cycles for MT3 with denaturation at 94 °C for 30 s, annealing at 63 °C for 1 min, and extension at 72 °C for 1 min; followed by final extension at 70 °C for 3 min. ..

    Lysis:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Synthesized:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Article Title: Direct reprogramming of mouse fibroblasts into self-renewable alveolar epithelial-like cells
    Article Snippet: Images were captured using a TCS SP5 (Leica, Wetzlar, Germany) every minute for 30 min. .. Total RNA was isolated from TTFs, MEFs, iPULs, AT2 organoids, and whole lungs using the RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized via reverse transcription of RNA using the ReverTra Ace qPCR RT master mix (TOYOBO, Osaka, Japan) and TaKaRa thermal cycler Dice Touch (Takara, Kusatsu, Japan). .. RNA was quantified using NanoDrop One (Thermo Fisher Scientific) and the amount of RNA input for cDNA synthesis was standardized within each experiment.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Article Title: Direct reprogramming of mouse fibroblasts into self-renewable alveolar epithelial-like cells
    Article Snippet: Images were captured using a TCS SP5 (Leica, Wetzlar, Germany) every minute for 30 min. .. Total RNA was isolated from TTFs, MEFs, iPULs, AT2 organoids, and whole lungs using the RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized via reverse transcription of RNA using the ReverTra Ace qPCR RT master mix (TOYOBO, Osaka, Japan) and TaKaRa thermal cycler Dice Touch (Takara, Kusatsu, Japan). .. RNA was quantified using NanoDrop One (Thermo Fisher Scientific) and the amount of RNA input for cDNA synthesis was standardized within each experiment.

    Article Title: EGFR-Targeted Extracellular Vesicles Potentiate Doxorubicin-Induced Apoptosis and Tumor Suppression in Colorectal Cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Ambion, Carlsbad, CA, USA). .. RNA concentration and quality were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with an A260/A280 ratio between 1.8 and 2.0 were used. cDNA synthesis was performed with 0.5 μg of total RNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Kita-Ku, Japan) in a 20 μL reaction volume on a Thermal Cycler Dice Touch (TAKARA, Sydney, Australia). .. Quantitative PCR was performed using SYBR qPCR Mix (TOYOBO, Osaka, Japan) on a Real-Time StepOnePlus System (Thermo Fisher Scientific, Waltham, MA, USA).

    Isolation:

    Article Title: Direct reprogramming of mouse fibroblasts into self-renewable alveolar epithelial-like cells
    Article Snippet: Images were captured using a TCS SP5 (Leica, Wetzlar, Germany) every minute for 30 min. .. Total RNA was isolated from TTFs, MEFs, iPULs, AT2 organoids, and whole lungs using the RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized via reverse transcription of RNA using the ReverTra Ace qPCR RT master mix (TOYOBO, Osaka, Japan) and TaKaRa thermal cycler Dice Touch (Takara, Kusatsu, Japan). .. RNA was quantified using NanoDrop One (Thermo Fisher Scientific) and the amount of RNA input for cDNA synthesis was standardized within each experiment.

    Reverse Transcription:

    Article Title: Direct reprogramming of mouse fibroblasts into self-renewable alveolar epithelial-like cells
    Article Snippet: Images were captured using a TCS SP5 (Leica, Wetzlar, Germany) every minute for 30 min. .. Total RNA was isolated from TTFs, MEFs, iPULs, AT2 organoids, and whole lungs using the RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized via reverse transcription of RNA using the ReverTra Ace qPCR RT master mix (TOYOBO, Osaka, Japan) and TaKaRa thermal cycler Dice Touch (Takara, Kusatsu, Japan). .. RNA was quantified using NanoDrop One (Thermo Fisher Scientific) and the amount of RNA input for cDNA synthesis was standardized within each experiment.

    Purification:

    Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells
    Article Snippet: .. Purified total RNA (1 μg) was subjected to cDNA synthesis using an amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX, USA) with a TaKaRa Thermal Cycler Dice Touch (TP350, TAKARA BIO INC., Otsu, Shiga, Japan). .. Gene expression was measured with a 7500 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using an AMPIGENE® qPCR Green Mix Hi-ROX (Enzo Life Sciences, Farmingdale, NY, USA) and primers ordered from Bioneer Corp. (Daejeon, Republic of Korea).

    Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells
    Article Snippet: .. Purified total RNA (1 μg) was subjected to cDNA synthesis using an amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX, USA) with a TaKaRa Thermal Cycler Dice Touch (TP350, TAKARA BIO INC., Otsu, Shiga, Japan). .. Gene expression was measured with a 7500 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using an AMPIGENE ® qPCR Green Mix Hi-ROX (Enzo Life Sciences, Farmingdale, NY, USA) and primers ordered from Bioneer Corp. (Daejeon, Republic of Korea).

    cDNA Synthesis:

    Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells
    Article Snippet: .. Purified total RNA (1 μg) was subjected to cDNA synthesis using an amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX, USA) with a TaKaRa Thermal Cycler Dice Touch (TP350, TAKARA BIO INC., Otsu, Shiga, Japan). .. Gene expression was measured with a 7500 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using an AMPIGENE® qPCR Green Mix Hi-ROX (Enzo Life Sciences, Farmingdale, NY, USA) and primers ordered from Bioneer Corp. (Daejeon, Republic of Korea).

    Article Title: EGFR-Targeted Extracellular Vesicles Potentiate Doxorubicin-Induced Apoptosis and Tumor Suppression in Colorectal Cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Ambion, Carlsbad, CA, USA). .. RNA concentration and quality were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with an A260/A280 ratio between 1.8 and 2.0 were used. cDNA synthesis was performed with 0.5 μg of total RNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Kita-Ku, Japan) in a 20 μL reaction volume on a Thermal Cycler Dice Touch (TAKARA, Sydney, Australia). .. Quantitative PCR was performed using SYBR qPCR Mix (TOYOBO, Osaka, Japan) on a Real-Time StepOnePlus System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells
    Article Snippet: .. Purified total RNA (1 μg) was subjected to cDNA synthesis using an amfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT, Barker, TX, USA) with a TaKaRa Thermal Cycler Dice Touch (TP350, TAKARA BIO INC., Otsu, Shiga, Japan). .. Gene expression was measured with a 7500 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) using an AMPIGENE ® qPCR Green Mix Hi-ROX (Enzo Life Sciences, Farmingdale, NY, USA) and primers ordered from Bioneer Corp. (Daejeon, Republic of Korea).

    Concentration Assay:

    Article Title: EGFR-Targeted Extracellular Vesicles Potentiate Doxorubicin-Induced Apoptosis and Tumor Suppression in Colorectal Cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Ambion, Carlsbad, CA, USA). .. RNA concentration and quality were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with an A260/A280 ratio between 1.8 and 2.0 were used. cDNA synthesis was performed with 0.5 μg of total RNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Kita-Ku, Japan) in a 20 μL reaction volume on a Thermal Cycler Dice Touch (TAKARA, Sydney, Australia). .. Quantitative PCR was performed using SYBR qPCR Mix (TOYOBO, Osaka, Japan) on a Real-Time StepOnePlus System (Thermo Fisher Scientific, Waltham, MA, USA).

    Spectrophotometry:

    Article Title: EGFR-Targeted Extracellular Vesicles Potentiate Doxorubicin-Induced Apoptosis and Tumor Suppression in Colorectal Cancer
    Article Snippet: Total RNA was extracted using TRIzol Reagent (Ambion, Carlsbad, CA, USA). .. RNA concentration and quality were assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and only samples with an A260/A280 ratio between 1.8 and 2.0 were used. cDNA synthesis was performed with 0.5 μg of total RNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Kita-Ku, Japan) in a 20 μL reaction volume on a Thermal Cycler Dice Touch (TAKARA, Sydney, Australia). .. Quantitative PCR was performed using SYBR qPCR Mix (TOYOBO, Osaka, Japan) on a Real-Time StepOnePlus System (Thermo Fisher Scientific, Waltham, MA, USA).



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